Cells were treated for 16 h with either solvent or 100 nM 1,25(OH)2D3

Cells were treated for 16 h with either solvent or 100 nM 1,25(OH)2D3. period of 240 min. Combined screening identified in each of these promoter regions a VDRE and reporter gene assays confirmed their functionality. Moreover, re-ChIP assays monitored simultaneous association of VDR with RXR, coactivator, mediator and RNA polymerase II proteins on these regions. … Continue reading Cells were treated for 16 h with either solvent or 100 nM 1,25(OH)2D3